# US 2020/0347376 A1 · METHOD OF PRODUCING RuBisCO PROTEIN FIBERS

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- Number: US 2020/0347376 A1
- Document ID: US20200347376A1
- Status: Published application
- Kind: A1
- Application number: 16/936,221
- Filed: 2020-07-22
- Published: 2020-11-05
- Earliest claimed: 2017-06-14
- Assignees: R.J. Reynolds Tobacco Company
- Applicants: R.J. Reynolds Tobacco Company
- Inventors: Andries Sebastian; John P. Mua; David Neil McClanahan; Sammy Eni Eni; Thaddeus J. Jackson
- Web page: https://patentgrep.com/patent/US20200347376A1

## CPC

- C12N 9/88: Lyases (4.)
- A23J 1/007: {from leafy vegetables, e.g. alfalfa, clover, grass}
- A23J 3/14: Vegetable proteins
- A23J 3/26: using extrusion or expansion
- A23J 3/34: using enzymes
- A24B 15/12: of reconstituted tobacco
- A24B 15/16: of tobacco substitutes
- A24B 15/24: by extraction; Tobacco extracts
- A24B 15/241: {Extraction of specific substances}
- C12Y 401/01039: Ribulose-bisphosphate carboxylase (4.1.1.39)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A24B: MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- C12Y: ENZYMES

## Abstract

Ribulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, for example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C., filtering the heated combination through an about 20 μm filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers.

## Claims

### Claim at position 1

1-17. (canceled)

### Claim 18

One or more ribulose-1,5-bisphosphate oxygenase protein fibers, produced by a process comprising the steps of:
extracting ribulose-1,5-bisphosphate oxygenase protein from tobacco;
feeding the extracted ribulose-1,5-bisphosphate oxygenase protein into an extruder;
adding a plasticizer to the extruder forming a ribulose-1,5-bisphosphate oxygenase protein mixture;
moving the ribulose-1,5-bisphosphate oxygenase protein mixture through one or more barrels of the extruder;
heating the ribulose-1,5-bisphosphate oxygenase protein mixture to up to about 140 degrees C.;
filtering the ribulose-1,5-bisphosphate oxygenase protein mixture with an about 20 μm filter; and
exiting the ribulose-1,5-bisphosphate oxygenase protein mixture through an orifice to shape the mixture as it exits the extruder forming the one or more ribulose-1,5-bisphosphate oxygenase protein fibers.

### Claim 19 (depends on 18)

The fibers of claim 18, wherein the one or more ribulose-1,5-bisphosphate oxygenase protein fibers are formed into a fabric selected from a group consisting of: woven fabric, non-woven fabric, knitted fabric, or a combination thereof.

### Claim 20 (depends on 18)

The fibers of claim 18, wherein the one or more ribulose-1,5-bisphosphate oxygenase protein fibers are incorporated into a biomedical device.

### Claim 21 (depends on 18)

The fibers of claim 18, where the one or more ribulose-1,5-bisphosphate oxygenase protein fibers are used to form one or more food-grade pouches.

### Claim 22 (depends on 18)

The fibers of claim 18, where the one or more ribulose-1,5-bisphosphate oxygenase protein fibers are used to form one or more tobacco pouches