Beta-galactosidase Mutant, and Preparation Method and Application Thereof
AssigneeJiangnan University
InventorsJing WU, Dan WU, Xin GAO
Life of this patent
Estimating the term…
Abstract
The present invention discloses a β-galactosidase mutant, and a preparation method and application thereof, belonging to the fields of gene engineering and enzyme engineering. Amino acids of specific sites in the β-galactosidase are mutated, the β-galactosidase is transferred into a recombinant bacterium, and enzymatic transformation is performed under optimized conditions, so that the yield of galactooligosaccharide produced by the mutant reaches 59.8%, which is increased by about 70% as compared with that of wild enzyme, thereby implementing the increase of the galactooligosaccharide yield. The present invention has very high industrialized application value.
Claims4
A gene coding a mutant β-galactosidase enzyme comprising a variant of the amino acid sequence of SEQ ID NO:2 with one or more mutations selected from N140C, F264W, F304Q, and W806F, wherein the enzyme catalyzes conversion of lactose to galactooligosaccharide (GOS).
Dependencies are inferred from the claim text.