US 2020/0347368 A1 · LIPASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

Abstract

The present invention relates to a variant of a parent lipase, wherein said variant has at least 60% but less than 100% sequence identity with SEQ ID NO: 2; has lipase activity; has substitutions corresponding to G91A and N92D in the lid region located from position G82 to position 1100 of SEQ ID NO: 2; has one or more additional substitutions in the lid region; and has at least mutations in positions corresponding to position T231 and position N233 of SEQ ID NO: 2. The present invention also relates to compositions, methods for cleaning a surface using a variant or a composition of the invention; polynucleotides encoding the variants; nucleic acid constructs, vectors, host cells comprising the polynucleotides; methods of using the variants; and methods for obtaining the lipase variant.

Claims19

Dependent claims

A variant of a parent lipase, wherein said variant has:

a) at least 60% but less than 100% sequence identity with SEQ ID NO:
2,
b) lipase activity;
c) substitutions corresponding to G91A and N92D in the lid region located from position G82 to position I100 of SEQ ID NO:
2;
d) one or more additional substitutions in the lid region;
and
e) at least mutations in positions corresponding to position T231, preferably T231R, and position N233, preferably N233R, of SEQ ID NO:
2.

6 more elements

Dependencies are inferred from the claim text.

Lipase variants and polynucleotides encoding same

Life of this patent

Lipase variants and polynucleotides encoding same

Life of this patent

Abstract

Claims19