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US 2020/0347360 A1Published application
Method for producing exosomes and exosomes obtained thereby
AssigneeTokyo Institute of Technology
InventorsFumi KANO, Masayuki MURATA, Yuki SONODA
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Abstract
The object of the present invention is to provide a method for incorporating an arbitrary protein, lipid, carbohydrate, or nucleic acid into an exosome. The object can be solved by a method for preparing an exosome, comprising the steps of: (a) adding a biological toxin having a perforating activity to a medium containing cells and incubating the mixture, (b) adding ATP and incubating the mixture, and (c) adding a medium containing calcium ion and incubating the mixture.
Claims8
A method for preparing an exosome, comprising the steps of:
- (a) adding a biological toxin having a perforating activity to a medium containing cell and incubating the mixture,
- (b) adding ATP and incubating the mixture, and
- (c) adding a medium containing calcium ion and incubating the mixture.
Dependencies are inferred from the claim text.