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US 2020/0347357 A1Published application
Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
AssigneePresident and Fellows of Harvard College
InventorsDouglas A. Melton, Mads Gurtler
Life of this patent
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Abstract
Disclosed herein are methods for generating SC-β cells using chemically defined, completely serum free media, and isolated populations of SC-β cells for use in various applications, such as cell therapy.
Claims30
A serum free in vitro composition that comprises a ROCK inhibitor and a plurality of pancreatic progenitor cells that express PDX1 and NKX6.1.
A method of in vitro cell differentiation, comprising:
- contacting an in vitro cell population comprising pancreatic progenitor cells that express PDX1 and NKX6.1 with a BMP signaling pathway inhibitor, a retinoic acid signaling pathway activator, a sonic hedgehog pathway inhibitor, a TGF-beta pathway inhibitor, and a ROCK inhibitor, wherein the population is in serum-free media;
- and wherein the method results in a plurality of the pancreatic progenitor cells in the population to differentiate into insulin-expressing cells.
Dependencies are inferred from the claim text.