Mold for producing hydrogel support for three-dimensional cell culture and method for producing hydrogel support using same

A method for producing a hydrogel support for a 3-dimensional (3D) cell culture, the method comprising:

• (A) preparing an upper mold comprising a base and a plurality of upper unit molds protruding downward from the base to form accommodating portions of a female type corresponding to shapes of hydrogel supports, having through holes at bottoms thereof, and patterned at a lower portion of the base;
• (B) preparing a lower mold where a plurality of lower unit molds are patterned, wherein the plurality of lower unit molds are formed in a female type to respectively accommodate the plurality of upper unit molds protruding downward and have sealed lower portions;
• (C) mounting the upper mold on the lower mold such that the plurality of upper unit molds are respectively inserted into the plurality of lower unit molds;
• (D) filling a gel-phase material in the accommodating portions of the upper mold;
• (E) coagulating the gel-phase material accommodated in the accommodating portions of the upper mold;
• (F) separating the upper mold from the lower mold;
• and
• (G) extracting completed hydrogel supports from the accommodating portions of the upper mold by inserting an ejecting unit into the through holes formed on the upper mold.