Marine Bacteria Formulation Useful in Aquaculture

A method of making a marine bacterial composition, comprising:

• (a) growing a population of heterotrophic marine bacteria to a high density in a medium containing one or more sources for multiple amino acids;
• and subsequently
• (b) adding to the bacteria-bearing medium both (i) water to dilute the source for amino acids to a lower concentration and (ii) a sufficient amount of salt to maintain a total salt concentration at or above 2.0%, wherein further bacterial growth is slowed down but bacterial vitality is preserved such that, after 7 days, at least about 50% of the bacteria population remain viable.

The method of claim 30, wherein step (b) comprises adding to the bacteria-bearing medium artificial seawater or natural seawater.

The method of claim 30, wherein the bacteria are probiotic or antibiotic.

A formulation comprising a population of Phaeobacter inhibens in an aqueous medium at a concentration of at least about 1×108 CFU/ml, the medium comprising a peptone or tryptone, yeast extract and natural or artificial sea water with a total salt concentration at about 2.0-3.5%, the peptone or tryptone at a concentration of no more than about 7.5 g/L, and the yeast extract at a concentration of no more than about 3.0 g/L.