A device for producing globules of adipose tissue, comprising a service unit and a single-use kit which may be housed in said service unit, said single-use kit comprising a working chamber, a circuit for introducing an adipose tissue sample into said working chamber, and a circuit for drawing globules of adipose tissue from said working chamber, said working chamber is formed by a flexible bag and in that said single-use kit further comprises a washing circuit introducing a saline solution washing said tissue sample into said working chamber, a size-reducing circuit which connects an exit route of said working chamber to an entry route of said working chamber, and a bypass circuit bypassing said size-reducing circuit, and recirculating said sample and said saline solution into said working chamber, said service unit having an electronic controller and a forced circulator forcing a circulation of said tissue sample through said size-reducing circuit, said size-reducing circuit comprising a mechanical size-reducing unit that does not require a supply of electricity to operate, said size reducing circuit further comprising a claiming element and a flexible tube comprising a first tube section that connects the exit route of the working chamber to an entry route of the size-reducing unit and a second, lower, lumen-shaped tube section which connects an exit route of the size-reducing unit to the entry route of the working chamber, said bypass circuit also comprising a flexible tube equipped with a claiming element, said flexible tube of said bypass circuit connecting the size-reducing circuit upstream of the size-reducing unit through a three-way fitting, a washing mode with said clamping element of said size-reducing circuit closed and said clamping element of said bypass circuit open being provided where said sample and said saline solution are simply recirculated, and a trituration mode with said clamping element of said size-reducing circuit open and said clamping element of said bypass circuit closed being provided where said sample is forced to pass into the size-reducing unit together with said saline solution.