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US 2020/0346208 A1Published application
Method and Kit for Preservation of Adipose Tissue Grafts
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorJ. Peter Rubin
Life of this patent
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Abstract
Methods, devices, and kits are provided for use in washing or cryopreserving live cells or tissue, such as fat graft tissue.
Claims25
A cell or tissue isolation, purification, and storage device comprising:
- a vessel having a first end and a second end defining an internal chamber divided by a filter that retains fat cells or tissue and having a volume between the second end and the filter of at least 3 mL;
- a first adaptor or connector, such as a Luer lock or slip connector, at the first end of the vessel defining a first opening;
- and a second adaptor or connector, such as a Luer lock or slip connector, at the second end of the vessel and defining a second opening.
-5. (canceled)
-9. (canceled)
A kit for use in isolating and storing cells, such as a fat graft, comprising:
- from two to 25 cell or tissue storage devices, each storage device comprising a vessel having a first end and a second end defining an internal chamber divided by a filter that retains fat cells or tissue and having a volume between the second end and the filter of at least 3 mL;
- a first adaptor or connector, such as a Luer lock or slip connector, at the first end of the vessel defining a first opening;
- and a second adaptor or connector, such as a Luer lock or slip connector, at the second end of the vessel and defining a second opening;
- and
- at least one vessel, optionally having an adaptor or connector, such as a Luer lock or slip connector, comprising an amount of cryoprotectant, such as dimethyl sulfoxide (DMSO), human seum albumin, trehalose, or a combination of two or more of any of the preceding, able to cryopreserve at least 3 mL of cells or tissue, such as an adipose graft.
2 more elements
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(canceled)
Dependencies are inferred from the claim text.