Multi-layer skin substitute products and methods of making and using the same

A method of making a skin substitute product, comprising the steps of:

• (a) optionally depositing a first (“hypodermis-like”) layer comprising live mammalian adipocytes (e.g., induced pre-adipocytes) in a first hydrogel carrier on a substrate;
• then
• (b) depositing a second (“dermis-like”) layer on said first layer when present (or on said substrate when said first layer is not present), said second layer comprising live mammalian fibroblast cells and live mammalian follicle dermal papilla cells in a second hydrogel carrier;
• and then
• (c) depositing a third (“epidermis-like”) layer on said second layer, said third layer comprising live mammalian keratinocytes and live mammalian melanocytes in a third hydrogel carrier.