Gene expression system for probiotic microorganisms
AssigneeZBiotics Company
InventorZachary ABBOTT
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Abstract
Provided herein are recombinant microorganisms that express a subject polypeptide. Microorganisms can comprise an expression construct comprising a flagellin promoter operatively linked with a heterologous nucleotide sequence encoding the subject polypeptide. The flagellin promoter sequence can comprise a genetic modification that reduces CsrA inhibition of translation. Microorganisms also can comprise a genetic modification that reduces FlgM inhibition of SigD initiation of transcription. The target polypeptide can be an aldehyde dehydrogenase. Such microorganisms are useful in the treatment of alcohol hangover.
Claims22
1-62. (canceled)
A recombinant bacterium, wherein the recombinant bacterium constitutively expresses an expression product encoded by a heterologous nucleic acid sequence, wherein the expression product is an aldehyde dehydrogenase (ALDH) enzyme that oxidizes acetaldehyde to acetate.
A recombinant B. subtilis bacterium, wherein the recombinant bacterium comprises a heterologous nucleic acid sequence encoding an expression product, wherein the expression product is an aldehyde dehydrogenase (ALDH) enzyme that oxidizes acetaldehyde to acetate.
A recombinant bacterium,
- wherein the recombinant bacterium comprises a heterologous nucleic acid sequence encoding an expression product, wherein the expression product is an aldehyde dehydrogenase (ALDH) enzyme that oxidizes acetaldehyde to acetate,
- wherein the nucleic acid sequence encoding the expression product is operatively linked with a flagellin gene promoter,
- wherein the flagellin gene promoter comprises a mutation in a CsrA binding site,
- wherein the mutation in the CsrA binding site inhibits binding of CsrA to mRNA transcripts encoding the ALDH enzyme but does not preclude expression of the ALDH enzyme, and
- wherein the recombinant bacterium comprises a mutation of an endogenous flgM gene that reduces inhibition of a sigma factor by FlgM.
A recombinant bacterium,
- wherein the recombinant bacterium comprises a heterologous nucleic acid encoding an expression product operatively linked with a flagellin gene promoter,
- wherein the flagellin gene promoter comprises a mutation in a CsrA binding site,
- wherein the mutation in the CsrA binding site inhibits binding of CsrA to mRNA transcripts encoding the expression product but does not preclude expression of the expression product, and
- wherein the recombinant bacterium comprises a mutation of an endogenous flgM gene that reduces inhibition of a sigma factor by FlgM.
Dependencies are inferred from the claim text.