Method for producing cell mass including pituitary tissue, and cell mass thereof
AssigneeSumitomo Chemical Company, Limited
InventorTokushige NAKANO
Life of this patent
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Abstract
The present invention aims to provide a method for efficiently producing a cell mass containing pituitary tissue from pluripotent stem cells. A method for producing a cell mass containing pituitary tissue, including the following steps (1) and (2): (1) a first step of suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance, (2) a second step of suspension-culturing the aggregate obtained in the first step in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, thereby obtaining a cell mass comprising pituitary tissue.
Claims20
A method for producing a cell mass comprising pituitary tissue, the method comprising:
- (1) suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance, and
- (2) suspension-culturing the cell aggregate formed in (1) in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, to obtain a cell mass comprising pituitary tissue.
A method for producing a cell mass comprising pituitary tissue, the method comprising:
- (a) culturing pluripotent stem cells in the absence of feeder cells and in a medium comprising 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining an undifferentiated state,
- (1) suspension-culturing the pluripotent stem cells, which were cultured in (a), to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance, and
- (2) suspension-culturing the cell aggregate formed in (1) in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, to obtain a cell mass comprising pituitary tissue.
A method for producing a cell mass comprising pituitary tissue, the method comprising:
- (1) suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance,
- (2-1) suspension-culturing the cell aggregate formed in (1) in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, and
- (2-2) suspension-culturing the cell aggregate suspension-cultured in (2-1) in the presence of a BMP signal transduction pathway inhibiting substance, to obtain a cell mass comprising pituitary tissue.
A method for producing a cell mass comprising pituitary tissue, the method comprising:
- (a) culturing pluripotent stem cells in the absence of feeder cells and in a medium comprising 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining an undifferentiated state,
- (1) suspension-culturing the pluripotent stem cells, which were cultured in (a), to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance,
- (2-1) suspension-culturing the cell aggregate formed in (1) in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, and
- (2-2) suspension-culturing the cell aggregate suspension-cultured in (2-1) in the presence of a BMP signal transduction pathway inhibiting substance, to obtain a cell mass comprising pituitary tissue.
A method for producing a pituitary tissue, the method comprising:
- (1) suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance,
- (2) suspension-culturing the cell aggregate formed in (1) in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance, to obtain a cell mass comprising pituitary tissue, and
- (3) collecting pituitary tissue from the cell mass obtained in (2).
A method for producing a pituitary tissue, the method comprising:
- (1) suspension-culturing pluripotent stem cells to form a cell aggregate in the presence of a Wnt signal transduction pathway inhibiting substance,
- (2-1) suspension-culturing the cell aggregate formed in (1) in the presence of a BMP signal transduction pathway activating substance and a Sonic hedgehog signal transduction pathway activating substance,
- (2-2) suspension-culturing the cell aggregate suspension-cultured in (2-1) in the presence of a BMP signal transduction pathway inhibiting substance, to obtain a cell mass comprising pituitary tissue, and
- (3) collecting pituitary tissue from the cell mass obtained in (2-2).
A cell mass comprising 1) neural cells or neural tissue, 2) pituitary tissue and 3) mesenchymal cells.
A cell mass comprising 1) neural cells or neural tissue, 2) pituitary tissue and 4) neural crest cells and neural crest-derived cells.
Dependencies are inferred from the claim text.