Artificial Vitreous Humor for the Investigation of Drugs and Drug Formulations
AssigneeHoffmann-La Roche Inc.
InventorsUlrike ALTENBURGER, Sulabh Pravinchandra PATEL, Gregoire SCHWACH, Pankaj SHENDE
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Abstract
The invention relates to an artificial vitreous humor composition comprising a phosphate buffer, wherein the phosphate buffer has a pH value in the range from 7.0 to 7.7, particularly from 7.1 to 7.6, more particularly from 7.2 to 7.5. The invention further relates to a method of production of an artificial vitreous humor composition, a method for analyzing the behavior of a substance, a method for analyzing the change of the artificial vitreous humor composition upon contact with a substance and a kit of parts.
Claims18
An artificial vitreous humor composition comprising a phosphate buffer, wherein the phosphate buffer has a pH value in the range from 7.0 to 7.7.
A method for the production of an artificial vitreous humor composition, comprising the steps
- (i) Providing stock solutions of glucose, creatinine, sodium lactate, glutathione and urea in water, of hypoxanthine in formic acid:water of 2:1 ratio, and of xanthine sodium in 1M NaOH;
- (ii) Mixing of stock solutions of step (i) in a phosphate buffer with a pH value in the range from 7.0 to 7.7, and pH adjustment to the pH value of the phosphate buffer;
- (iii) Addition of hyaluronic acid to the mixture obtained in step (iii) and stirring at 2 to 8° C. at least for 4 hours;
- (iv) Addition of collagen type-II to the mixture obtained in step (iii);
- (v) Optionally sterile-filtering of the mixture obtained in step (iv).
A kit of parts for the production of an artificial vitreous humor composition, comprising
- (A) a phosphate buffer, having a pH value in the range from 7.0 to 7.7;
- (B) at least one selected from the group consisting of creatinine, glucose, urea, xanthine, hypoxanthine, sodium lactate, glutathione;
- (C) type II collagen and/or sodium hyaluronate;
- and optionally
- (D) instructions for use.
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Dependencies are inferred from the claim text.