Tissue engineering meniscal composite scaffold and preparation method thereof

A method for preparing a tissue engineering meniscal composite scaffold comprising:

• a modeling step to generate a three-dimensional data model of the meniscus to be regenerated before damage;
• a printing step, in which a scaffold is printed according to the three-dimensional data model using degradable polymer as raw material, and the scaffold is C-shaped and has a shape which is consistent with the original shape of the meniscus to be regenerated, the scaffold comprises a plurality of first degradable polymer fibers extending along the circumferential direction of the scaffold and a plurality of second degradable polymer fibers extending along the radial direction of the scaffold;
• the first degradable polymer fibers form a multilayer intersection with the second degradable polymer fibers and thereby generating a frame structure having a plurality of first apertures, the diameter of the first apertures is 750 μm-1500 μm;
• a hydrophilic treatment step, in which the scaffold is subjected to hydrophilic treatment;
• a preparation step for preparing lyophilized meniscal composite scaffold, in which a solution comprising matrix material is filled into the plurality of first apertures of the scaffold and the scaffold is subjected to lyophilization to obtain the lyophilized meniscal composite scaffold;
• a post-processing step, in which the lyophilized meniscal composite scaffold is subjected to cross-linking treatment and sterilization treatment to obtain a meniscal composite scaffold having a plurality of second apertures, the diameter of the second apertures is 90 μm-150 μm.