Cassette device for quick test of diagnosis, method for detecting a ligand in a biological sample and kit

A device for rapid diagnostic test containing a lower cassette containing recesses where a test strip containing one or more of a sample ligand and a positive control ligand of reaction characterized for comprising:

• a lower cassette (1) containing recesses where a test strip (2) containing one or more of a sample ligand and a positive control ligand of reaction is positioned;
• a first absorbing pad (3) positioned over the test strip, upstream thereof;
• a second absorbing pad (4) positioned over the test strip (2), downstream thereof;
• a capsule (5) containing buffer upstream the test strip (2), and a spear-shaped structure (6) located in a position anterior the capsule (5),
• an upper cassette (7) having a capillary-tube-shaped structure (8) to be filled with a blood sample, located over the absorbing pad (3) upstream, and that is fitted to the lower cassette (1) forming an assembly containing an inner cavity isolated from external environment, where the rapid test reaction occurs,
• a compressible, movable cover (9) that is connected to the lower cassette (1) and to the upper cassette (7) upstream the capillary-shaped structure (8), promoting assembly closure, wherein compressing the movable cover (9) causes the spear-shaped structure (6) to press the capsule (5) containing buffer, such that said capsule (5) is disrupted, pouring the buffer solution over the absorbing pad (3) located upstream the test strip (2), going through entire test strip (2) until it is absorbed by the absorbing pad (4) located downstream the test strip (2), and,
• wherein compressing the movable cover (9) promotes capillary-tube-shaped structure closure (8).