Pegylated antifreeze proteins and methods of making and using the same
OwnerYong BA, Mohammad SALAMEH +1inventors
InventorsYong BA, Mohammad SALAMEH, Adiel PEREZ
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Abstract
The present disclosure concerns a modified antifreeze protein having the formula AFP-PEG, where AFP is an antifreeze protein, PEG is a poly(alkylene glycol) unit, and the PEG is linked to an amino acid residue in the AFP that is not involved in direct ice-surface binding and that has a functional group selected from an amine, a thiol, a hydroxy, a carboxylate, an amide and a guanidine in its side chain. A formulation including the same, a method of protecting a biological tissue, organ or body using the same, and a method of synthesizing a modified antifreeze protein are also disclosed.
Claims27
A modified antifreeze protein having the formula AFP-PEG, wherein:
- a) AFP is an antifreeze protein;
- b) PEG is a poly(alkylene glycol) unit;
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- c) the PEG is linked to an amino acid residue in the AFP that is not involved in direct ice-surface binding and that has a functional group selected from an amine, a thiol, a hydroxy, a carboxylate, an amide and a guanidine in its side chain.
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A method of synthesizing a modified antifreeze protein, comprising:
- a) reacting an antifreeze protein (AFP) having an amino acid residue with a functional group selected from an amine, a thiol, a hydroxy, a carboxylate, an amide and a guanidine in its side chain that is not involved in direct ice-surface binding with a functionalized poly(alkylene glycol) having a weight average molecular weight or number average molecular weight of from 0.3 to 20 kDa, the functionalized poly(alkylene glycol) containing a reacting group capable of reacting with the amine, thiol, hydroxy, carboxylate, amide or guanidine functional group to form the modified AFP;
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- b) purifying the modified AFP.
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Dependencies are inferred from the claim text.