Loading patent…
AssigneeAkeagen, Inc.
InventorsLibin CUI, Xiaolin SUN
Estimating the term…
The present application provides genetically modified non-human animals and methods for producing heavy chain-only antibodies (HcAbs), wherein the genetically modified non-human animal comprises a germline genome comprising an engineered immunoglobulin heavy chain (IgH) allele at an endogenous IgH locus, wherein the engineered IgH allele lacks functional gene segments encoding CH1 domains of all endogenous IgG subclasses. In some embodiments, a genetically modified mouse is provided, comprising an engineered IgH allele that lacks a functional endogenous gene segment encoding Cγ3, Cγ1, Cγ2b and CH1 exon of Cγ2c. Further provided are HcAbs or derivatives thereof produced by the genetically modified non-human animals.
1-33. (canceled)
A method of producing a heavy chain-only antibody (HcAb) that specifically binds to an antigen of interest, comprising:
A heavy chain-only antibody (HcAb) isolated from a genetically modified non-human animal, wherein the genetically modified non-human animal comprises a germline genome comprising an engineered immunoglobulin heavy chain (IgH) allele at an endogenous IgH locus, wherein the engineered IgH allele lacks endogenous gene segments encoding functional CH1 domains of all endogenous IgG subclasses.
40-42. (canceled)
A B cell isolated from a genetically modified non-human animal of, wherein the genetically modified non-human animal comprises a germline genome comprising an engineered immunoglobulin heavy chain (IgH) allele at an endogenous IgH locus, wherein the engineered IgH allele lacks endogenous gene segments encoding functional CH1 domains of all endogenous IgG subclasses.
(canceled)
Dependencies are inferred from the claim text.