Methods for quantitating protein biomarkers
OwnerBaochuan Guo, Ravali Algandulainventors
Life of this patent
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Abstract
Disclosed are methods of detecting biomarkers in complex biological matrices. The method comprises providing a sample well coated with antibodies specific to target protein biomarker(s), contacting the sample well with a biological sample to capture target biomarkers, digesting target biomarkers, and analyzing the signature peptides resulting from digestion of target biomarkers to determine the amount of one or more protein biomarkers by a mass spectrometry method.
Claims27
A method of determining the amount of a biomarker in a biological sample, the method comprising:
- providing a sample well coated with an antibody specific to a target biomarker;
- contacting the sample well with a biological sample to form a bound sample;
- digesting the bound sample to form a digested sample;
- and analyzing the digested sample to determine the amount of one or more target biomarkers.
The method of claim 11, wherein the second protein is bovine serum albumin.
A method of determining the amount of a protein biomarker in a biological sample, the method comprising:
- providing a sample well coated with at least one antibody specific to a target protein biomarker;
- contacting the sample well with a biological sample to form a bound sample;
- eluting the bound sample;
- adding a second protein to the bound sample;
- digesting the bound sample in the presence of urea in a concentration of about 8M and trypsin to form a digested sample;
- and analyzing the digested sample to determine the amount of one or more protein biomarkers by comparison to a tryptic peptide produced by trypsin digestion of a protein biomarker to an isotope labeled peptide standard;
- wherein the digested sample is analyzed by mass spectrometry.
A method of screening for colorectal cancer, the method comprising:
- providing a sample well coated with an antibody specific to hemoglobin;
- contacting the sample well with a stool sample to form a bound sample;
- digesting said bound sample to form a digested sample;
- and analyzing said digested sample to determine the amount of hemoglobin in the stool sample.
Dependencies are inferred from the claim text.