Sample preparation and flow-through sensors using functionalized silicon nanomembranes

A method of preparing, detecting, or assaying an analyte of a sample, comprising:

• contacting the sample with a fluidic device comprising a functionalized silicon membrane, wherein the fluidic device isolates one or more analyte of interest from the sample;
• passing a wash solution through the fluidic device;
• and
• i) eluting the isolated analyte of interest;
• transferring the eluted analyte of interest to a storage vessel or analytical instrument;
• and
• performing one or more analytical assays on the eluted analyte of interest;
• or
• ii) passing a solution of one or more detection reagent through the fluidic device;
• optionally, passing additional wash solution through the fluidic device;
• and
• measuring a signal of one or more detection reagent;
• or
• iii) extracting nucleic acids from the analyte captured by the fluidic device;
• performing a sequencing and/or amplification reaction, wherein reagents for such reactions are passed into the fluidic device;
• optionally, passing a second wash solution through the fluidic device;
• optionally, passing a solution of one or more detection reagent through the device;
• measuring a signal of one or more amplification and/or sequencing reaction products.