Method for Producing Nicotinamide Mononucleotide and Transformant Used in Said Method
AssigneeMitsubishi Chemical Corporation
InventorTakanori Akiyama
Life of this patent
Estimating the term…
Abstract
An object of the present invention is to provide a method for producing nicotinamide mononucleotide (NMN) with excellent production efficiency. The method for producing NMN according to the present invention (the first aspect) comprises the step of bringing a transformant with enhanced expression of enzymes nicotinamide phosphoribosyltransferase (Nampt), phosphoribosyl pyrophosphate synthetase (Prs) and polyphosphate kinase (Ppk), a cell-free protein synthesis reaction solution having the three enzymes expressed, or a treated product thereof into contact with a mixture containing ribose-5-phosphate (R5P), nicotinamide (NAM), ATP and polyphosphate.
Claims17
A method for producing nicotinamide mononucleotide (NMN), comprising the step of bringing a transformant with enhanced expression of three enzymes nicotinamide phosphoribosyltransferase (Nampt), phosphoribosyl pyrophosphate synthetase (Prs) and polyphosphate kinase (Ppk), a cell-free protein synthesis reaction solution having the three enzymes expressed, or a treated product thereof into contact with ribose-5-phosphate (R5P), nicotinamide (NAM), ATP and polyphosphate.
A transformant with enhanced expression of three enzymes nicotinamide phosphoribosyltransferase (Nampt), phosphoribosyl pyrophosphate synthetase (Prs) and polyphosphate kinase (Ppk).
A transformant with enhanced expression of two enzymes ribokinase (Rbk) and polyphosphate kinase (Ppk).
A method for producing nicotinamide mononucleotide (NMN), comprising the step of bringing a transformant with enhanced expression of nicotinamide phosphoribosyltransferase (Nampt), a cell-free protein synthesis reaction solution having the enzyme expressed, or a treated product thereof into contact with nicotinamide (NAM) and phosphoribosyl pyrophosphate (PRPP) in the presence of pyrophosphatase (PPase).
A method for producing NMN, comprising the step of bringing a transformant with enhanced expression of nicotinamide phosphoribosyltransferase (Nampt) or a treated product thereof into contact with at least nicotinamide (NAM), wherein the transformant has disruption or deletion of a gene encoding an enzyme classified into EC number represented by (d) EC 3.5.1.42, and a gene encoding an enzyme classified into at least any one of EC numbers represented by the following (a), (c), (g), (h) and (i):
- (a) EC 3.1.3.5,
- (c) EC 2.4.2.1,
- (g) EC 3.2.2.1,
- (h) EC 3.2.2.3, and
- (i) EC 3.2.2.14.
A method for producing nicotinamide mononucleotide (NMN), comprising the step of bringing a transformant with enhanced expression of two enzymes nicotinamide phosphoribosyltransferase (Nampt) and phosphoribosyl pyrophosphate synthetase (Prs), a cell-free protein synthesis reaction solution having the two enzymes expressed, or a treated product thereof into contact with ribose-5-phosphate (R5P), nicotinamide (NAM) and ATP, wherein the total sum of the respective numbers of moles of ATP, ADP and AMP to be added to the reaction system is 0.5 equivalents or less of the number of moles of NMN to be generated.
Dependencies are inferred from the claim text.