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US 2020/0329685 A1Published application
Transgenic eukaryotic organisms and methods for gender selection
AssigneeRamot at Tel-Aviv University Ltd.
InventorsEhud QIMRON, Ido YOSEF, Mordechay GERLIC
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Abstract
The present invention provides systems and non-invasive methods for gender selection of eukaryotic organisms More specifically, the invention applies the CRISPR-Cas system as well as any derivatives and fusion proteins thereof for creation of transgenic eukaryotic organisms and for selecting the desired gender of the resulting progeny.
Claims21
1-46. (canceled)
A system comprising:
- (A) a transgenic eukaryotic heterogametic organism comprising one of:
- (a) at least one nucleic acid sequence:
- (i) said sequence encoding or forming at least one target recognition element for at least one nucleic acids modifier protein;
- or
- (ii) said sequence encoding or forming said at least one target recognition element and a nucleic acid sequence encoding a first fragment, domain or subunit of at least one said nucleic acids modifier protein;
- or
- (b) at least one nucleic acid sequence encoding:
- (i) at least one nucleic acids modifier protein;
- or
- (ii) a second fragment, domain or subunit of said at least one nucleic acids modifier protein;
- wherein said nucleic acid sequence is integrated into one of the gender-chromosomes of said transgenic heterogametic organism;
- and
- (B) a transgenic eukaryotic homogametic organism comprising one of:
- (a) at least one nucleic acid sequence encoding:
- (i) at least one nucleic acids modifier protein;
- or
- (ii) a second fragment, domain or subunit of said at least one nucleic acids modifier protein;
- or
- (b) at least one nucleic acid sequence:
- (i) said sequence encoding or forming at least one target recognition element for at least one nucleic acids modifier protein;
- or
- (ii) said sequence encoding or forming said at least one target recognition element and a nucleic acid sequence encoding a first fragment, domain or subunit of at least one said nucleic acids modifier protein;
- wherein said nucleic acid sequence is integrated into at least one allele of any chromosomal or mitochondrial DNA of said transgenic homogametic organism.
20 more elements
A method for selecting a desired gender of an eukaryotic organism, the method comprising the steps of:
- (A) providing a transgenic eukaryotic heterogametic organism comprising one of:
- (a) at least one nucleic acid sequence:
- (i) said sequence encoding or forming at least one target recognition element for at least one nucleic acids modifier protein;
- or
- (ii) said sequence encoding or forming said at least one target recognition element and a nucleic acid sequence encoding a first fragment, domain or subunit of at least one said nucleic acids modifier protein;
- or
- (b) at least one nucleic acid sequence encoding:
- (i) at least one nucleic acids modifier protein;
- or
- (ii) a second fragment, domain or subunit of said at least one nucleic acids modifier protein;
- wherein said nucleic acid sequence is integrated into one of the gender-chromosomes of said transgenic heterogametic organism;
- (B) providing a transgenic eukaryotic homogametic organism comprising one of:
- (a) at least one nucleic acid sequence encoding:
- (i) at least one nucleic acids modifier protein;
- or
- (ii) a first fragment, domain or subunit of said at least one nucleic acids modifier protein;
- or
- (b) at least one nucleic acid sequence:
- (i) said sequence encoding or forming at least one target recognition element for at least one nucleic acids modifier protein;
- or
- (ii) said sequence encoding or forming said at least one target recognition element and a nucleic acid sequence encoding a first fragment, domain or subunit of at least one said nucleic acids modifier protein;
- wherein said nucleic acid sequence is integrated into at least one allele of any chromosomal or mitochondrial DNA of said transgenic homogametic organism;
- and
- (C) breeding said transgenic heterogametic organism provided in step (A) with said transgenic homogametic organism provided in step (B), thereby obtaining a progeny predominantly composed of said one desired gender.
21 more elements
A method for reducing the population of an eukaryotic species comprising the steps of:
- (a) providing a transgenic heterogametic organism of said species comprising:
- (i) at least one nucleic acid sequence encoding at least one guide RNA directed against at least one target sequence within at least one of coding and non-coding sequences, or any product/s thereof, of at least one chromosome of said organism;
- wherein said nucleic acid sequence is integrated into one of the gender-chromosomes of said transgenic heterogametic organism;
- and
- (ii) at least one nucleic acid sequence encoding at least one guide RNA directed against at least one gene, or any product/s thereof, encoding a product essential for fertility;
- wherein said nucleic acid sequence is integrated into the other gender-chromosomes of said transgenic heterogametic organism;
- (b) providing a transgenic eukaryotic homogametic organism comprising at least one nucleic acid sequence encoding at least one RNA guided DNA binding protein nuclease, wherein said nucleic acid sequence is integrated into at least one allele of any chromosome of said transgenic homogametic organism;
- (c) breeding said transgenic heterogametic organism provided in step (a) with said transgenic homogametic organism provided in step (b), thereby obtaining a sterile progeny predominantly composed of said one desired gender;
- and
- (d) releasing said sterile progeny obtained in step (c) into the wild;
- optionally, wherein said eukaryotic species is an insect, and wherein said insect is a mosquito and the progeny obtained in step (c) is a sterile male.
8 more elements
A transgenic eukaryotic heterogametic organism or any progeny, or a transgenic eukaryotic homogametic organism, or any cell or product thereof, wherein:
- (I) said heterogametic organism comprises one of:
- (a) at least one nucleic acid sequence:
- (i) said sequence encoding or forming at least one target recognition element for at least one nucleic acids modifier protein;
- or
- (ii) said sequence encoding or forming said at least one target recognition element and a nucleic acid sequence encoding a first fragment, domain or subunit of at least one said nucleic acids modifier protein;
- or
- (b) at least one nucleic acid sequence encoding:
- (i) at least one nucleic acids modifier protein;
- or
- (ii) a second fragment, domain or subunit of said at least one nucleic acids modifier protein;
- wherein said nucleic acid sequence is integrated into one of the gender-chromosomes of said transgenic heterogametic organism;
- or
- (II) said homogametic organism comprises one of:
- (a) at least one nucleic acid sequence encoding:
- (i) at least one nucleic acids modifier protein;
- or
- (ii) a second fragment, domain or subunit of said at least one nucleic acids modifier protein;
- or
- (b) at least one nucleic acid sequence:
- (i) said sequence encoding or forming at least one target recognition element for at least one nucleic acids modifier protein;
- or
- (ii) said sequence encoding or forming said at least one target recognition element and a nucleic acid sequence encoding a first fragment, domain or subunit of at least one said nucleic acids modifier protein;
- wherein said nucleic acid sequence is integrated into at least one allele of any chromosomal or mitochondrial DNA of said transgenic homogametic organism.
20 more elements
Dependencies are inferred from the claim text.