Mapping the Location, Type and Strand of Damaged and/or Mismatched Nucleotides in Double-Stranded DNA

A kit comprising:

• (a) a nucleotide mix comprising one or more modified nucleotides;
• (b) a ligase;
• and
• (c) one or a plurality of enzymes that provide nick translating activity, wherein the nucleotide mix, ligase and one or a plurality of enzymes are contained in a single container or reaction vessel or in one or more different containers or reaction vessels.

A composition comprising:

• (a) one or more nucleic acid repair enzymes;
• (b) a nucleotide mix comprising one or more modified nucleotides;
• (c) a ligase;
• and
• (d) a polymerase capable of nick translating activity.

A method for detecting a damaged site in a double-stranded nucleic acid derived from a genomic nucleic acid, comprising:

• (a) creating a nick at the damaged site;
• (b) performing a nick translating reaction from the nick using a nucleotide mix comprising one or more modified nucleotides;
• (c) sealing the nick-translated DNA in the double-stranded DNA with a ligase to form a double-stranded DNA product comprising a plurality of said modified nucleotides;
• and
• (d) detecting the plurality of the modified nucleotides in the double-stranded DNA product, wherein said plurality of modified nucleotides correspond to the damaged site.