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AssigneeThe Governing Council of the University of Toronto
InventorsRadhakrishnan Mahadevan, Alexander Yakunin, Pratish Gawand, Kayla Nemr
Estimating the term…
A non-naturally occurring microorganism having a 1,3-BDO pathway is provided. The microorganism expresses at least one of the following 1,3-BDO pathway enzymes: an aldolase that catalyzes condensation of two acetaldehydes to produce 3-hydroxybutanal; and an aldo-ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase that reduces 3-hydroxybutanal to 1,3-BDO. The organism may further express one or more enzymes for producing acetaldehyde. A biosynthetic process involves condensing two acetaldehyde molecules to 3-hydroxybutanal using an enzyme from class aldolases; and selectively reducing 3-hydroxybutanal to 1,3-BDO using an enzyme belonging to the class aldo-ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase. The process can further include producing acetaldehyde by a biosynthetic method.
1-25. (canceled)
A non-naturally occurring microorganism that produces 1,3-butanediol (1,3-BDO) from 3-hydroxybutanal, the microorganism being engineered to express an exogenous polynucleotide encoding an aldo-keto reductase (AKR) comprising an amino acid sequence at least 85% identical to SEQ ID NO:
A non-naturally occurring microorganism that produces 1,3-BDO from 3-hydroxybutanal, the microorganism being engineered to express an exogenous polynucleotide encoding an enzyme that catalyzes reduction of the 3-hydroxybutanal to 1,3-BDO, wherein said enzyme is a variant of a Pseudomonas aeruginosa AKR, said Pseudomonas aeruginosa AKR comprising an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:
A method for producing 1,3-BDO from 3-hydroxybutanal, the method comprising contacting 3-hydroxybutanal with an aldo-keto reductase (AKR) comprising an amino acid sequence at least 85% identical to SEQ ID NO:
Dependencies are inferred from the claim text.