Winter Aconite Fatty Acid Elongase And Uses Thereof In The Production Of Fatty Acids
AssigneeNational Research Council of Canada
InventorsDauenpen MEESAPYODSUK, Xiao QIU, Bob CHAPMAN
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Abstract
Fatty acids, and methods for the production thereof, are provided. Transgenic organisms, microbes, plants, seeds, and cells useful in the production of fatty acids, along with related expression vectors, phages, plasmids, nucleic acids, and enzymes, are also provided. Methods for the production of fatty acids such as docosadienoic acid and docosatrienoic acid, involving the use of winter aconite (Eranthis hyemalis) EhELO1 elongase, are described in detail.
Claims35
An isolated nucleic acid molecule encoding an elongase enzyme, said nucleic acid molecule comprising:
- i) a nucleotide sequence having at least 70% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 5;
- ii) a codon degenerate nucleotide sequence of SEQ ID NO:
- 5;
- iii) a nucleotide sequence as set forth in SEQ ID NO:
- 6;
- iv) a nucleotide sequence encoding a polypeptide having at least 85% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 4;
- or
- v) a nucleotide sequence encoding a polypeptide having a conservatively substituted amino acid sequence of SEQ ID NO:
- 4.
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Dependencies are inferred from the claim text.