Method for producing alkaline phosphatase, alkaline phosphatase obtained using said method, and vector and transformant for production thereof

A vector for Aspergillus transformation, comprising a nucleotide sequence encoding an ALP, wherein the nucleotide sequence includes a gene bound to a 5′-end side of the nucleotide sequence, the gene comprising any of following nucleotide sequences (a) to (c):

• (a) a nucleotide sequence set forth in any of SEQ ID NOs:
• 2 to 15;
• (b) a nucleotide sequence consisting of a nucleotide sequence formed from a nucleotide sequence set forth in any of SEQ ID NOs:
• 2 to 15 with deletion, substitution, or addition of one or several nucleotides and encoding a secretory signal peptide having ALP secretion activity;
• and
• (c) a nucleotide sequence having a homology of 90% or higher with a nucleotide sequence set forth in any of SEQ ID NOs:
• 2 to 15 and encoding a secretory signal peptide having ALP secretion activity;
• or
• a gene consisting of a nucleotide sequence encoding any of the following secretory signal peptides (d) to (f):
• (d) a secretory signal peptide consisting of an amino acid sequence set forth in any of SEQ ID NOs:
• 17 to 30;
• (e) a secretory signal peptide consisting of an amino acid sequence formed from an amino acid sequence set forth in any of SEQ ID NOs:
• 17 to 30 with deletion, substitution, or addition of one or several amino acids and having ALP secretion activity;
• and
• (f) a secretory signal peptide having a homology of 90% or higher with an amino acid sequence set forth in any of SEQ ID NOs:
• 17 to 30 and having ALP secretion activity.