Method of producing lactic acid bacteria dual-coated with protein and polysaccharide by using protein hydrolysate
AssigneeCell Biotech Co., Ltd.
InventorMyung Jun CHUNG
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Abstract
The present disclosure relates to a method of producing lactic acid bacteria dual-coated with protein and polysaccharide by using a protein hydrolysate, and lactic acid bacteria having a dual coating, produced by the method. The lactic acid bacteria having a dual coating of protein and polysaccharide, produced according to the present disclosure, have very excellent dry-freezing viability, acid resistance and bile resistance. Accordingly, the lactic acid bacteria having a dual coating of protein and polysaccharide according to the present disclosure will be very useful for the production of fermented milk, processed milk, fermented soy products, processed foods, functional beverages, functional foods, common foods, etc.
Claims20
A method of producing lactic acid bacteria having a dual coating of protein and polysaccharide coatings, the method comprising the steps of:
- (a) treating an aqueous protein solution with a protease, thereby preparing an aqueous protein hydrolysate solution having a protein hydrolysis rate of 45% to 95%;
- (b) adding a sugar component and a nitrogen source component for lactic acid bacteria culture to the prepared aqueous protein hydrolysate solution, followed by sterilization, and then inoculating and culturing the lactic acid bacteria in the sterilized aqueous solution;
- (c) recovering lactic acid bacteria cells from a fermented lactic acid bacteria culture obtained by the culturing;
- (d) adding an aqueous cryoprotectant solution and an aqueous polysaccharide solution to the recovered lactic acid bacteria cells, followed by mixing and homogenization;
- and
- (e) freeze-drying the homogenized aqueous lactic acid bacteria cell solution.
Dependencies are inferred from the claim text.