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AssigneeJiangnan University
InventorsJuan ZHANG, Xueliang QIU, Jianghua LI, Guocheng DU, Jian CHEN
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The present disclosure discloses recombinant Escherichia coli and application thereof in screening erythritol-producing strains, and belongs to the technical field of microorganisms. The recombinant Escherichia coli used in a method for screening an erythritol-producing strain disclosed by the present disclosure can well perform positive correlation induction on erythritol with different concentrations, so that the method for screening the erythritol-producing strain has the advantage of high sensitivity. High-concentration glucose is usually adopted as a fermentation substrate when erythritol is produced in a fermentation mode in the industry, but the method for screening the erythritol-producing strain disclosed by the present disclosure can overcome the interference of the high-concentration glucose, and under the interference of the high-concentration glucose, the recombinant Escherichia coli used in the method for screening the erythritol-producing strain can still well perform positive correlation induction on erythritol with different concentrations, and the correlation is higher than that without the interference of the glucose. Therefore, the method for screening the erythritol-producing strain has the advantage of strong anti-interference capability.
A recombinant plasmid, wherein the recombinant plasmid comprises a recombinant pET-22b(+) plasmid as an expression vector with insertion of a gene encoding a transcriptional regulation factor and a marker gene, and the nucleotide sequence of the gene encoding the transcriptional regulation factor is set forth as SEQ ID No.1.
Dependencies are inferred from the claim text.