Coated Substrate for Biological Reaction Systems
AssigneeLife Technologies Corporation
InventorsEliodor GHENCIU, Theodore E. STRAUB, Evan W. FOSTER, Michael C. PALLAS
Life of this patent
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Abstract
An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
Claims22
An apparatus for biological reactions, the apparatus comprising:
- a substrate;
- and
- a plurality of reaction sites within the substrate,
- wherein a surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume,
- wherein the sample volume of each loaded reaction site is substantially confined to its respective reaction site, and
- wherein the sample volume is configured to undergo a biological reaction within the reaction site.
(canceled)
(canceled)
A method for designing a substrate with a plurality of reaction sites, the method comprising:
- determining an advancing water contact angle of a liquid sample with a first material;
- determining a receding water contact angle for a liquid sample with the first material;
- and
- if the receding water contact angle is within 15 degrees of the advancing water contact angle, coating a first surface of a substrate and a surface of each reaction site of the plurality of reaction sites with the first material.
An instrument, comprising:
- a detection system;
- control system configured to control the functions of the detection system;
- a sample support device including substrate comprising a substrate surface and a plurality of reaction sites disposed along the substrate surface, each reaction site comprising a reaction site surface;
- a coating material, wherein:
- the coating material coats the substrate surface, to provide a coated substrate surface;
- the coating material coats each of the reaction site surfaces to provide a plurality of coated reaction sites;
- the coated substrate surface has a water contact angle of 60-90 degrees;
- and
Dependencies are inferred from the claim text.