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US 2020/0319171 A1Published application
Composite particles, coated particles, method for producing composite particles, ligand-containing solid phase carrier and method for detecting or separating target substance in sample
AssigneesJSR Micro N.V.C., Jsr Corporation, Jsr Life Sciences Corporation, JSR Micro N.V.
InventorsTakeaki MASUDA, Shunsuke Onogi
Life of this patent
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Abstract
The present invention relates to composite particles, coated particles, a method of producing composite particles, a ligand-containing solid phase carrier, and a method of detecting or separating a target substance in a sample. The above described composite particles each contains an organic polymer and inorganic nanoparticles, wherein the content of the inorganic nanoparticles in the composite particles is more than 80% by mass, and wherein the composite particles have a volume average particle size of from 10 to 1,000 nm.
Claims20
1:
- Composite particles, each comprising an organic polymer and inorganic nanoparticles,
- wherein a content of the inorganic nanoparticles in the composite particles is more than 80% by mass, and
- wherein the composite particles have a volume average particle size of from 10 to 1,000 nm.
2:
- The composite particles according to claim 1, wherein the composite particles have a coefficient of variation of the volume average particle size of 20% or less.
3:
- The composite particles according to claim 1, wherein the content of the inorganic nanoparticles is from more than 80% by mass to 95% by mass or less.
4:
- The composite particles according to claim 1, wherein the inorganic nanoparticles have a volume average particle size of from 5 to 25 nm.
5:
- The composite particles according to claim 1, wherein the composite particles comprise at least one selected from the group consisting of a surfactant, an acid group-containing compound, an amino group-containing compound, a silane group-containing compound and a titanium atom-containing compound.
6:
- The composite particles according to claim 1, wherein the inorganic nanoparticles are particles capable of being magnetized.
7:
- The composite particles according to claim 1, wherein the inorganic nanoparticles comprise:
- at least one inorganic material selected from the group consisting of a simple substance of iron, titanium, cobalt, zinc, copper, manganese, nickel, gadolinium, an oxide thereof, and an alloy thereof;
- and
- a ferrite.
1 more element
8:
- The composite particles according to claim 1, wherein the inorganic nanoparticles are metal oxide particles.
9:
- The composite particles according to claim 1, wherein the composite particles are magnetic particles.
10:
- The composite particles according to claim 1, wherein the composite particles have surfaces to which at least one ligand can be physically adsorbed.
11:
- The composite particles according to claim 1, wherein the composite particles have surfaces to which at least one ligand can be chemically bound.
12:
- Coated particles comprising:
- the composite particles according to claim 1;
- and
- a polymer layer coating the composite particles.
1 more element
13:
- A method of producing composite particles, the method comprising:
- mixing a magnetic fluid, a monomer and a polymerization initiator to prepare a monomer mixed liquid;
- dispersing the monomer mixed liquid to prepare an emulsion;
- and
- polymerizing the monomer in the emulsion.
2 more elements
14:
- A ligand-containing solid phase carrier, which is a solid phase carrier selected from the coated particles according to claim 12, and which comprises a ligand.
15:
- The ligand-containing solid phase carrier according to claim 14, wherein the ligand is at least one selected from the group consisting of an antibody, an antigen, a nucleic acid, a nucleotide, a nucleoside, a protein, a peptide, an amino acid, a polysaccharide, a saccharide, a lipid, a vitamin, a drug, a substrate, a hormone and a neurotransmitter.
16:
- An immunoassay or a method for detecting a nucleic acid, comprising employing the ligand-containing solid phase carrier according to claim 14.
17:
- A method of detecting or separating a target substance in a sample, the method comprising contacting the sample with the ligand-containing solid phase carrier according to claim 14.
18:
- A ligand-containing solid phase carrier, which is a solid phase carrier selected from the composite particles according to claim 1, and which comprises a ligand.
19:
- The ligand-containing solid phase carrier according to claim 18, wherein the ligand is at least one selected from the group consisting of an antibody, an antigen, a nucleic acid, a nucleotide, a nucleoside, a protein, a peptide, an amino acid, a polysaccharide, a saccharide, a lipid, a vitamin, a drug, a substrate, a hormone and a neurotransmitter.
20:
- An immunoassay or a method for detecting a nucleic acid, comprising employing the ligand-containing solid phase carrier according to claim 18.