Methods and genetically modified cells for production of fatty acid esters
AssigneeCargill, Incorporated
InventorsMichael Tai Man LOUIE, Ana NEGRETE-RAYMOND, Travis Robert WOLTER
Life of this patent
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Abstract
A recombinant cell for producing a fatty acid ester. The recombinant cell is genetically engineered to produce a reduction in free fatty acids compared to a cell that has not been similarly genetically engineered. Methods for producing fatty acid esters while decreasing free fatty acid production are also described.
Claims180
A recombinant cell for producing a fatty acid ester, wherein the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 1-3,
- wherein the recombinant cell is capable of producing at least 5 g/L total mono fatty acid ester.
The recombinant cell of any one of the preceding claims, wherein the recombinant cell is a fungal cell, or a bacterial cell.
The recombinant cell of any one of the preceding claims, wherein the recombinant cell is Escherichia coli.
The recombinant cell of any one of the preceding claims, wherein the recombinant cell is yeast.
A method for decreasing free fatty acid production, the method comprising culturing a recombinant cell, wherein:
- the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 1-3;
- and
- the recombinant cell is capable of producing at least 5 g/L fatty acid ester.
A recombinant cell for producing a fatty acid ester, wherein the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 4,
- wherein the recombinant cell is capable of producing at least 5 g/L total fatty acid ester.
A method for decreasing free fatty acid production, the method comprising culturing a recombinant cell, wherein:
- the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 4;
- and
- the recombinant cell is capable of producing at least 5 g/L total fatty acid ester.
A recombinant cell for producing a fatty acid ester, wherein the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 4 and for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 5,
- wherein the recombinant cell is capable of producing at least 5 g/L total fatty acid ester.
A method for decreasing free fatty acid production in a recombinant cell, the method comprising culturing a recombinant cell, wherein:
- the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 4 and for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 5;
- and
- the recombinant cell is capable of producing at least 5 g/L total fatty acid ester.
A recombinant cell for producing a fatty acid ester, wherein the recombinant cell is genetically engineered for increased activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 7,
- wherein the recombinant cell is capable of producing at least 5 g/L fatty acid ester.
A method for decreasing free fatty acid production, the method comprising culturing a recombinant cell, wherein:
- the recombinant cell is genetically engineered for increased activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 7;
- wherein the recombinant cell produces a reduction in total free fatty acids compared to a recombinant cell without increased activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 7;
- and
- the recombinant cell is capable of producing at least 5 g/L total fatty acid ester.
A recombinant cell for producing a fatty acid ester, wherein the recombinant cell is genetically engineered for increased activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 7,
- wherein the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 4 and for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 5,
- wherein the recombinant cell is capable of producing at least 5 g/L total fatty acid ester.
A method for decreasing free fatty acid production, the method comprising culturing a recombinant cell, wherein:
- the recombinant cell is genetically engineered for increased activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 7;
- the recombinant cell is genetically engineered for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 4 and for reduced activity of a polypeptide with at least 80%, 85%, 90%, 95%, 98%, or 100% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 5;
- and
- the recombinant cell is capable of producing at least 5 g/L of total fatty acid ester.
Dependencies are inferred from the claim text.