Carotenoid production method
AssigneeJxtg Nippon Oil & Energy Corporation
InventorsHidetada NAGAI, Wataru SATO, Toshiyuki TAKAHASHI, Harumi SATO
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Abstract
A mutant carotenoidogenic bacterium, comprising any of genes (a)-(c) below: (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 225th amino acid residue in the amino acid sequence of 1-deoxy-D-xylulose 5-phosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; (b) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 305th amino acid residue in the amino acid sequence of decaprenyl diphosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; and (c) both of the genes (a) and (b) above.
Claims22
A mutant carotenoidogenic bacterium, comprising any of genes (a)-(c) below:
- (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 225th amino acid residue in the amino acid sequence of 1-deoxy-D-xylulose 5-phosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue;
- (b) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 305th amino acid residue in the amino acid sequence of decaprenyl diphosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue;
- and
- (c) both of the genes (a) and (b) above.
A method for screening for a carotenoidogenic bacterium, comprising subjecting a carotenoidogenic bacterium to a mutation treatment, and selecting a bacterium having any of characteristics (a)-(c) below from the bacteria subjected to the mutation treatment:
- (a) a characteristic where the activity of 1-deoxy-D-xylulose 5-phosphate synthase is increased compared to said activity in the bacterium before the mutation treatment;
- (b) a characteristic where the activity of decaprenyl diphosphate synthase is decreased compared to said activity in the bacterium before the mutation treatment;
- and
- (c) both of the characteristics (a) and (b) above.
A gene encoding a protein comprising a mutant amino acid sequence in which at least the 225th amino acid residue in the amino acid sequence of 1-deoxy-D-xylulose 5-phosphate synthase has been substituted with other amino acid residue.
A gene comprising either of DNA (a) or (b) below:
- (a) DNA comprising the nucleotide sequence represented by SEQ ID NO:5;
- or
- (b) DNA that hybridizes with DNA having a nucleotide sequence complementary to said DNA (a) under stringent conditions, and that encodes a protein having 1-deoxy-D-xylulose 5-phosphate synthase activity.
A gene encoding a protein comprising a mutant amino acid sequence in which at least the 305th amino acid residue in the amino acid sequence of decaprenyl diphosphate synthase has been substituted with other amino acid residue.
A gene comprising either of DNA (a) or (b) below:
- (a) DNA comprising the nucleotide sequence represented by SEQ ID NO:7;
- or
- (b) DNA that hybridizes with DNA having a nucleotide sequence complementary to said DNA (a) under stringent conditions, and that encodes a protein with decreased decaprenyl diphosphate synthase activity.
Dependencies are inferred from the claim text.