Cellobiohydrolase Variants and Polynucleotides Encoding Same
AssigneeNovozymes A/S
InventorsMark Wogulis, Leslie DeMars, David Osborn
Life of this patent
Estimating the term…
Abstract
The present invention relates to cellobiohydrolase variants having increased thermal activity or thermostability, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims20
A cellobiohydrolase variant, comprising a substitution at one or more positions corresponding to positions 8, 17, 113, 157, 159, 184, 199, 240, 250, 274, 318, 325, 328, 347, 349, 358, 360, 380, 391, 393, 394, 412, and 430 of SEQ ID NO:
- 1, wherein the variant has cellobiohydrolase activity, and wherein the variant has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to a parent cellobiohydrolase.
A cellobiohydrolase variant, comprising a variant catalytic domain, wherein the variant catalytic domain comprises a substitution at one or more positions corresponding to positions 8, 17, 113, 157, 159, 184, 199, 240, 250, 274, 318, 325, 328, 347, 349, 358, 360, 380, 391, 393, 394, 412, and 430 of SEQ ID NO:
- 1 and has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the catalytic domain of a parent cellobiohydrolase.
A method for obtaining a cellobiohydrolase variant, comprising introducing into a parent cellobiohydrolase a substitution at one or more positions corresponding to positions 8, 17, 113, 157, 159, 184, 199, 240, 250, 274, 318, 325, 328, 347, 349, 358, 360, 380, 391, 393, 394, 412, and 430 of the polypeptide of SEQ ID NO:
- 1, wherein the cellobiohydrolase variant has cellobiohydrolase activity;
- and recovering the variant.
Dependencies are inferred from the claim text.