Engineered bacterial ribosome compositions and methods
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsSidney Hecht, Larisa Dedkova, Shengxi Chen, Xiaoguang Bai
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Abstract
Compositions and methods relating to bacterial ribosomes selected to increase the incorporation of at least one glycosylated amino acid into a protein versus a wild-type bacterial ribosome. Selection embodiments include growing bacteria in the presence of a puromycin derivative, wherein a surviving clone has a ribosome that incorporates at least one glycosylated amino acid into a protein.
Claims20
A composition comprising a bacterial ribosome selected to increase the incorporation of a glycosylated amino acid into a protein versus a wild-type bacterial ribosome, wherein the bacterial ribosome has been selected with a puromycin derivative.
A method for selecting a ribosomal clone configured to incorporate at least one glycosylated amino acid into a protein, comprising the step of growing bacteria in the presence of a puromycin derivative and selecting a clone, wherein the surviving clone comprises a ribosome that incorporates at least one glycosylated amino acid into a protein.
A method for synthesizing a glycosylated protein with a selected bacterial ribosomal clone grown in the presence of a puromycin derivative and thereby configured to incorporate at least one glycosylated amino acid into a protein, comprising the step of adding an S-30 extract from the selected bacterial ribosomal clone to an in vitro translation system under conditions and for a time suitable to synthesize a glycosylated protein.
Dependencies are inferred from the claim text.