Microfluidic paper chip for detecting micro-organism, method for preparing the same and method for detecting micro-organism using the same
AssigneeBiomax Co., Ltd.
InventorsChan Ho KWON, Jae Hoon KIM
Life of this patent
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Abstract
Disclosed is a microfluidic paper chip for detecting a microorganism, a method for preparing the same and a method for detecting the microorganism using the same. The microfluidic paper chip includes a lysis layer and a chromogenic layer which are laminated in this order. The lysis layer is composed of a hydrophilic paper containing a lysis reagent composition and the chromogenic layer is composed of a hydrophilic paper containing a chromogenic reagent.
Claims13
A microfluidic paper chip for detecting a microorganism, the microfluidic paper chip comprising a lysis layer and a chromogenic layer which are laminated in this order, wherein
- the lysis layer is composed of a hydrophilic paper containing a lysis reagent composition and
- the chromogenic layer is composed of a hydrophilic paper containing a chromogenic reagent.
A method for producing a microfluidic paper chip, the method comprising:
- (a) a step of forming a hydrophobic barrier wall by printing a hydrophobic material on the edges of multiple papers made of a hydrophilic material;
- (b) a step of making a lysis reagent composition absorbed into a hydrophilic area of one of the papers on which the hydrophobic material is printed and then performing drying;
- (c) a step of making a chromogenic reagent absorbed into a hydrophilic area of another one of the papers on which the hydrophobic material is printed and then performing drying;
- and
- (d) a step of sequentially laminating the paper on which the hydrophobic material is printed, the paper on which said lysis reagent composition is printed, the paper on which said chromogenic reagent is printed, and the paper on which said hydrophobic material is printed.
Dependencies are inferred from the claim text.