Anti-inflammatory compositions, methods and uses thereof
AssigneeApimed Medical Honey Limited
InventorsJennifer KUEHNE, Gregor Aaron STEINHORN, John Alexander TAYLOR
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Abstract
Described herein are anti-inflammatory fractions and compounds, medicaments, methods and uses thereof. The fraction may be obtained from honey. The fraction shows a generalised anti-inflammatory response. Also described are method f producing the fraction as well as a method of testing the anti-inflammatory potential of a honey by completing the fractionation and a cell test.
Claims16
A method of treating a skin condition on a subject in need thereof by the step of administering a medicament to an affected skin site on a subject in need thereof, the medicament having anti-inflammatory activity and comprising as an active, a less than or equal to 10 kDa molecular weight fraction obtained from honey using a less than or equal to 10 kDa filter.
A method of treating a stomach ulcer and/or a digestive tract condition in a subject in need thereof by the step of administering a medicament to the subject, the medicament having anti-inflammatory activity and comprising as an active, a less than or equal to 10 kDa molecular weight fraction obtained from honey using a less than or equal to 10 kDa filter.
10-18. (canceled)
A method of producing an anti-inflammatory activity fraction comprising a less than or equal to 10 kDa fraction from honey, the method comprising:
- (a) selecting a honey;
- (b) diluting the honey in an aqueous solution and filtering the diluted honey via a less than or equal to 10 kDa filter;
- (c) collecting a less than or equal to 10 kDa fraction wherein this fraction has anti-inflammatory activity.
A method of testing the anti-inflammatory potential of a honey by the steps of:
- (a) stimulating a cell line with a suitable stimulant to induce an inflammatory response in the cells;
- (b) preparing a <10 kDa fraction from a honey by diluting the honey sample and collecting a <10 kDa fraction by subjecting the diluted honey to a filtration separation step;
- (c) applying the filtered honey containing the <10 kDa fraction to the inflamed cells;
- (d) measuring the reduction in cytokine production and/or nitrogen oxide production to determine the anti-inflammatory potential of the honey sample wherein the greater the reduction in inflammation as measured via cytokine production and/or nitric oxide production, the greater the anti-inflammatory activity of the honey tested.
Dependencies are inferred from the claim text.