Match-paired Monoclonal Antibodies against MRJP4, ELISA Kit and Colloidal Gold Immunoassay Test Strip for Detecting MRJP4

An enzyme-linked immunosorbent assay kit for detecting a content of MRJP4, comprising:

• a primary antibody against the MRJP4, a biotin-labeled secondary antibody against the MRJP4, a standard, a horseradish peroxidase-labeled avidin, a biotin-labeled antibody diluent, a horseradish peroxidase-labeled avidin diluent, a sample diluent, a concentrated washing solution, a substrate solution, and a stop solution;
• wherein
• the primary antibody is a first monoclonal antibody against the MRJP4 and the biotin-labeled secondary antibody is a second monoclonal antibody against the MRJP4;
• or the primary antibody is the second monoclonal antibody against the MRJP4and the biotin-labeled secondary antibody is the first monoclonal antibody against the MRJP4;
• wherein the first monoclonal antibody against the MRJP4 is secreted by a hybridoma cell line 6H9 having a microbial deposit number of CGMCC No. 17294 and the second monoclonal antibody against the MIUP4 is secreted by a hybridoma cell line 8C9 having a microbial deposit number of CGMCC No. 17295.

A colloidal gold immunoassay test strip for detecting MIUP4, comprising:

• a gold colloidal conjugate pad, a nitrocellulose (NC) membrane containing a detecting line and a quality controlling line, a sample pad, a water absorbing pad, and a detecting bottom plate;
• wherein,
• the gold colloidal conjugate pad comprises a conjugate of a first monoclonal antibody and a colloidal gold;
• the NC membrane comprises one detecting line and one quality controlling line;
• the detecting line is composed of a second monoclonal antibody, and the quality controlling line is composed of a goat anti-mouse immunoglobulin G (IgG) antibody;
• or
• the gold colloidal conjugate pad comprises a conjugate of the second monoclonal antibody and a colloidal gold;
• the NC membrane comprises one detecting line and one quality controlling line;
• the detecting line is composed of the first monoclonal antibody, and the quality controlling line is composed of a goat anti-mouse immunoglobulin G (IgG) antibody;
• wherein the first monoclonal antibody is secreted by a hybridoma cell line 6H9 having a microbial deposit number of CGMCC No. 17294 and the second monoclonal antibody is secreted by a hybridoma cell line 8C9 having a microbial deposit number of CGMCC No. 17295.