Back to results
US 2020/0292540 A1Published application
Reversible surface functionalization
AssigneeIllumina Cambridge Limited
InventorsXavier von Hatten, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Andrew A. Brown
Life of this patent
Estimating the term…
Abstract
Some examples described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
Claims20
A method for reversibly immobilizing a biological molecule to a surface of a substrate, comprising:
- contacting a plurality of host molecules with functionalized silane covalently attached thereto the surface of the substrate, wherein the functionalized silane comprises a plurality of hydrophobic guest moieties, and the plurality of hydrophobic guest moieties comprise aryl, heteroaryl, heterocyclyl, cycloalkyl, cycloalkenyl, or cycloalkynyl, or optionally substituted variants thereof;
- wherein each host molecule comprises a biological molecule covalently bonded thereto;
- and
- wherein the biological molecule is immobilized to the surface of the substrate through a host-guest reversible interaction between the hydrophobic guest moiety of the functionalized silane and the host molecule.
1 more element
A substrate comprising a plurality of biological molecules immobilized to a surface of the substrate,
- wherein each of the plurality of biological molecules is covalently bonded to a host molecule comprising a hydrophobic portion and a hydrophilic portion;
- wherein the surface of the substrate comprises functionalized silane covalently attached thereto, and the functionalized silane comprises a plurality of hydrophobic guest moieties;
- and
- wherein each of the plurality of biological molecules is immobilized through a host-guest interaction between the hydrophobic guest moiety of the functionalized silane and the host molecule.
1 more element
Dependencies are inferred from the claim text.