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US 2020/0291366 A1Published application
Pross optimized enzymes
AssigneeIgc Bio, Inc.
InventorsLior ZIMMERMAN, Dror BARAN
Life of this patent
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AssigneeIgc Bio, Inc.
InventorsLior ZIMMERMAN, Dror BARAN
Estimating the term…
The present invention provides enzymes that have been optimized by implementation of Protein Repair One Stop Shop (PROSS), an algorithm that generates protein design(s) for enhanced stability without changing either enzymatic properties or enzyme active site conformation of the respective enzyme. The protein design(s) generated by PROSS introduce mutations to the amino acid sequence of a wild-type protein, resulting in a mutated amino acid sequence that encodes a variant of the wild-type enzyme, i.e., “an enzyme variant”, which has an enhanced stability, core packing, surface polarity and backbone rigidity, a higher functional expression, and/or a combination thereof, compared to the stability core packing, surface polarity and backbone rigidity, functional expression and/or a combination thereof, of the wild-type enzyme.
A pyruvate transaminase variant of a wild-type pyruvate transaminase from Vibrio fluvialis of SEQ ID NO:
A pyruvate transaminase variant of a wild-type pyruvate transaminase from Vibrio fluvialis of SEQ ID NO:
A triacylglycerol lipase variant of a wild-type triacylglycerol lipase from Burkholderia glumae of SEQ ID NO:
A triacylglycerol lipase variant of a wild-type triacylglycerol lipase from Burkholderia glumae of SEQ ID NO:
A halohydrin dehalogenase variant of a wild-type halohydrin dehalogenase from Rhizobium radiobacter of SEQ ID NO:
A halohydrin dehalogenase variant of a wild-type halohydrin dehalogenase from Rhizobium radiobacter of SEQ ID NO:
A aminotransferase variant of a wild-type aminotransferase from Chromobacterium violaceum of SEQ ID NO:
A aminotransferase variant of a wild-type aminotransferase from Chromobacterium violaceum of SEQ ID NO:
A putative secreted lipase variant of a wild-type putative secreted lipase from Clostridium botulinum of SEQ ID NO:
A putative secreted lipase variant of a wild-type putative secreted lipase from Clostridium botulinum of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Geobacillus stearothermophilus of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Geobacillus stearothermophilus of SEQ ID NO:
A histidine acid phosphatase variant of a wild-type histidine acid phosphatase from Yersinia mollaretii of SEQ ID NO:
A histidine acid phosphatase variant of a wild-type histidine acid phosphatase from Yersinia mollaretii of SEQ ID NO:
A chanoclavine-I aldehyde reductase easA variant of a wild-type chanoclavine-I aldehyde reductase easA from Neosartorya fumigata of SEQ ID NO:
A chanoclavine-I aldehyde reductase easA variant of a wild-type chanoclavine-I aldehyde reductase easA from Neosartorya fumigata of SEQ ID NO:
An alanine dehydrogenase variant of a wild-type alanine dehydrogenase from Archaeoglobus fulgidus of SEQ ID NO:
An alanine dehydrogenase variant of a wild-type alanine dehydrogenase from Archaeoglobus fulgidus of SEQ ID NO:
An acid phosphatase variant of a wild-type acid phosphatase from Shimwellia blattae of SEQ ID NO:
An acid phosphatase variant of a wild-type acid phosphatase from Shimwellia blattae of SEQ ID NO:
A major phosphate-irrepressible acid phosphatase variant of a wild-type major phosphate-irrepressible acid phosphatase from Morganella morganii of SEQ ID NO:
A major phosphate-irrepressible acid phosphatase variant of a wild-type major phosphate-irrepressible acid phosphatase from Morganella morganii of SEQ ID NO:
An acid phosphatase variant of a wild-type acid phosphatase from Prevotella intermedia of SEQ ID NO:
An acid phosphatase variant of a wild-type acid phosphatase from Prevotella intermedia of SEQ ID NO:
A tryptophan dimethylallyltransferase variant of a wild-type tryptophan dimethylallyltransferase from Neosartorya fumigata of SEQ ID NO:
A tryptophan dimethylallyltransferase variant of a wild-type tryptophan dimethylallyltransferase from Neosartorya fumigata of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Geobacillus stearothermophilus of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Geobacillus stearothermophilus of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Rhodococcus jostii of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Rhodococcus jostii of SEQ ID NO:
An acid phosphatase variant of a wild-type acid phosphatase from Shigella flexneri of SEQ ID NO:
An acid phosphatase variant of a wild-type acid phosphatase from Shigella flexneri of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Aromatoleum aromaticum of SEQ ID NO:
An alcohol dehydrogenase variant of a wild-type alcohol dehydrogenase from Aromatoleum aromaticum of SEQ ID NO:
An arylesterase variant of a wild-type arylesterase from Pseudomonas pseudoalcaligenes of SEQ ID NO:
An arylesterase variant of a wild-type arylesterase from Pseudomonas pseudoalcaligenes of SEQ ID NO:
A putrescine aminotransferase variant of a wild-type putrescine aminotransferase from Escherichia coli of SEQ ID NO:
A putrescine aminotransferase variant of a wild-type putrescine aminotransferase from Escherichia coli of SEQ ID NO:
A green to red photoconvertible GFP-like protein EosFP variant of a wild-type green to red photoconvertible GFP-like protein EosFP from Lobophyllia hemprichii of SEQ ID NO:
A green to red photoconvertible GFP-like protein EosFP variant of a wild-type green to red photoconvertible GFP-like protein EosFP from Lobophyllia hemprichii of SEQ ID NO:
A red fluorescent protein drFP583 variant of a wild-type red fluorescent protein drFP583 from Discosoma sp. of SEQ ID NO:
A red fluorescent protein drFP583 variant of a wild-type red fluorescent protein drFP583 from Discosoma sp. of SEQ ID NO:
A green fluorescent protein variant of a wild-type green fluorescent protein from Aequorea victoria of SEQ ID NO:
A green fluorescent protein variant of a wild-type green fluorescent protein from Aequorea victoria of SEQ ID NO:
A luciferin 4-monooxygenase variant of a wild-type luciferin 4-monooxygenase from Photinus pyralis of SEQ ID NO:
A luciferin 4-monooxygenase variant of a wild-type luciferin 4-monooxygenase from Photinus pyralis of SEQ ID NO:
A mOrange variant of a wild-type mOrange from Discosoma sp. of SEQ ID NO:
A mOrange variant of a wild-type mOrange from Discosoma sp. of SEQ ID NO:
A sandercyanin fluorescent protein variant of a wild-type sandercyanin fluorescent protein from Sander vitreus of SEQ ID NO:
A sandercyanin fluorescent protein variant of a wild-type sandercyanin fluorescent protein from Sander vitreus of SEQ ID NO:
A GFP-like fluorescent chromoprotein FP538 variant of a wild-type GFP-like fluorescent chromoprotein FP538 from Zoanthus sp. of SEQ ID NO:
A GFP-like fluorescent chromoprotein FP538 variant of a wild-type GFP-like fluorescent chromoprotein FP538 from Zoanthus sp. of SEQ ID NO:
An mNeonGreen variant of a wild-type mNeonGreen from Branchiostoma lanceolatum of SEQ ID NO:
An mNeonGreen variant of a wild-type mNeonGreen from Branchiostoma lanceolatum of SEQ ID NO:
A green fluorescent protein blFP-Y3 variant of a wild-type green fluorescent protein blFP-Y3 from Branchiostoma lanceolatum of SEQ ID NO:
A green fluorescent protein blFP-Y3 variant of a wild-type green fluorescent protein blFP-Y3 from Branchiostoma lanceolatum of SEQ ID NO:
A far-red fluorescent protein eqFP650 variant of a wild-type far-red fluorescent protein eqFP650 from Entacmaea quadricolor of SEQ ID NO:
A far-red fluorescent protein eqFP650 variant of a wild-type far-red fluorescent protein eqFP650 from Entacmaea quadricolor of SEQ ID NO:
A fluorescent protein cyOFP variant of a wild-type fluorescent protein cyOFP from Escherichia coli of SEQ ID NO:
A fluorescent protein cyOFP variant of a wild-type fluorescent protein cyOFP from Escherichia coli of SEQ ID NO:
Dependencies are inferred from the claim text.