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AssigneeRubius Therapeutics, Inc.
InventorsTiffany Fen-Yi Chen, Thomas Joseph Wickham, Christopher Lawrence Moore, Shamael Rabia Dastagir, Douglas Charles Mclaughlin, Regina Sophia Salvat, Alex Richard Nanna
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The present disclosure provides customizable enucleated erythroid cells or enucleated cells that can be engineered to include, on their surface, a loadable exogenous antigen-presenting polypeptide, wherein the loadable exogenous antigen-presenting polypeptide comprises one or more amino acid substitutions. In some embodiments, the one or more amino acid substitutions stabilize the loadable exogenous antigen-presenting polypeptide on the cell surface. In some embodiments, the loadable exogenous antigen-presenting polypeptide is stabilized on the cell surface in the absence of a polypeptide bound to the loadable exogenous antigen-presenting polypeptide. In some embodiments, the loadable exogenous antigen-presenting polypeptide comprises an exogenous displaceable polypeptide bound to the loadable exogenous antigen-presenting polypeptide. In some embodiments, the loadable exogenous antigen-presenting polypeptide is stabilized on the cell surface upon release of the displaceable polypeptide.
An engineered enucleated erythroid cell comprising a loadable exogenous antigen-presenting polypeptide on its cell surface, wherein the loadable exogenous antigen-presenting polypeptide comprises one or more amino acid substitutions which stabilize the loadable exogenous antigen-presenting polypeptide on the cell surface.
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A method of treating a subject in need of an altered immune response, the method comprising:
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A method of making an engineered enucleated erythroid cell comprising an antigen-loaded exogenous antigen-presenting polypeptide, the method comprising:
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A method of making an engineered enucleated erythroid cell comprising a loadable exogenous antigen-presenting polypeptide on its cell surface, the method comprising:
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Dependencies are inferred from the claim text.