Chlamydia-activated b cell platforms and methods thereof
AssigneeOhio State Innovation Foundation
InventorsThomas Cherpes, Rodolfo Vicetti Miguel
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Abstract
Disclosed herein is a Chlamydia-activated B cell (CAB) platform. Also disclosed is a method of enhancing a population of B cells, comprising exposing said B cells to Chlamydia spp. under conditions suitable to enhance the population of B cells, such that expansion and differentiation of said B cells takes place, and said B cells are exposed or crosslinked to an antigen. Also disclosed are methods of producing said CABs, and treating a subject in need thereof with said CABs.
Claims58
A platform for creating activated, antigen-presenting cells (APCs), wherein the platform comprises:
- a. Chlamydia spp., or an activating protein, peptide, or fragment thereof;
- b. a population of B cells;
- and
- c. an antigen, wherein the antigen is not derived from a Chlamydia spp.
A method for producing activated, antigen-presenting Chlamydia-activated B cells in a subject, the method comprising
- a. obtaining B cells from a subject;
- b. exposing the B cells from step a) to Chlamydia spp., or an activating protein, peptide, or fragment thereof;
- c. exposing the B cells of step b) to a desired antigen, wherein the antigen is not derived from Chlamydia spp., thereby obtaining activated, antigen-presenting Chlamydia-activated B cells (CABs)
A method of treating a subject in need thereof, the method comprising
- a. obtaining B cells from the subject;
- b. exposing the B cells from step a) to Chlamydia spp., or an activating protein, peptide, or fragment thereof,
- c. exposing the B cells of step b) to an antigen, wherein the antigen is not derived from Chlamydia spp., thereby obtaining activated, antigen-presenting Chlamydia-activated B cells (CABs);
- and
- d. treating the subject with the activated, antigen-presenting Chlamydia-activated B cells of step c).
A method for producing activated, antigen-presenting cells in a subject, the method comprising:
- a. obtaining immunogenic cells, and
- b. crosslinking an antigen to the immunogenic cell, thereby producing an activated, antigen-presenting cell.
Dependencies are inferred from the claim text.