Rapid thermal cycling for sample analyses and processing
AssigneeStar Array Pte Ltd
InventorsHAIQING GONG, WEN YAN, XUDONG ZENG
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Abstract
Method and apparatus for thermal processing of nucleic acid in a thermal profile is provided. The method employs at least a first bath and a second bath, the method further employing a reactor holder for holding reactor(s) accommodating reaction material containing the nucleic acid. The method comprises maintaining bath mediums in the baths at two different temperatures; and alternately allowing the reactor(s) to be in the two baths in a plurality of thermal cycles to alternately attain a predetermined high target temperature THT, and a predetermined low target temperature TLT, wherein the bath medium in at least one of the baths is a high thermal conductivity powder.
Claims44
Method for thermal processing of nucleic acid in a thermal profile, the method employing at least a first bath and a second bath, the method further employing a reactor holder for holding reactor(s) accommodating reaction material containing the nucleic acid and the reactor(s) being in any form such as tubes or wellplates or chips or cartridges, the method comprising:
- maintaining bath mediums in the baths at two different temperatures;
- and
- alternately allowing the reactor(s) to be in the two baths in a plurality of thermal cycles to alternately attain:
- a predetermined high target temperature THT, and
- a predetermined low target temperature TLT,
- wherein the bath medium in at least one of the baths is a high thermal conductivity powder.
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Apparatus for thermal processing of nucleic acid in a thermal profile, the apparatus employing a reactor holder for holding reactor(s) to accommodate reaction material containing nucleic acid and the reactor(s) being in any form such as tube(s) or wellplate(s) or chip(s) or cartridge(s), the apparatus comprising:
- a first bath;
- a second bath, bath mediums in the baths being respectively maintainable at two different temperatures, and
- a transfer means for allowing the reactor(s) to be in the two baths in a plurality of thermal cycles to alternately attain:
- a predetermined high target temperature THT, and
- a predetermined low target temperature TLT;
- and
- an amount of high thermal conductivity powder to serve as the bath medium in at least one of the baths.
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Dependencies are inferred from the claim text.