US 2020/0271671 A1 · IN-VITRO METHOD AND KIT FOR DIAGNOSING THE RISK OF SUFFERING FROM CARIES

Abstract

The invention describes a method of predicting the risk of caries in a subject and a kit based on said method, characterized in that the method involves the following steps: (a) determining the concentration in the saliva of the subject of: (i) at least one compound selected from among the components of the saliva of the immune system; (ii) at least one compound selected from among those involved in microbial adhesion to a surface of the buccal cavity; and (iii) at least one compound selected from among the components involved in the metabolism or regulation of pH in the buccal cavity; (b) comparing a response variable calculated on the basis of the at least one of the concentrations determined in (i), at least one of the concentrations determined in (ii), and at least one of the concentrations determined in (iii) in step (a) with a corresponding reference response variable in a population of subjects without caries or in a population of subjects with caries; and (c) determining the risk of caries on the basis of the degree of difference in the response variable compared in step (b), wherein a higher degree of difference between the response variable of the subject and the corresponding reference response variable in a population of subjects without caries or in a population of subjects with caries is indicative of a higher risk of caries in said subject.

Claims11

Dependent claims

A method for predicting caries risk in a subject, characterized in that said method comprises the following steps:

(a) determining the concentration in saliva from said subject of
i) at least one compound selected from salivary components of the immune system selected from the list consisting of Cathelicidin LL-37, IgA and Beta-Defensin-2;
and
ii) at least one compound selected from salivary components involved in microbial adhesion to a surface of the buccal cavity selected from the list consisting of Statherin, Fibronectin and Collagen I;
and
iii) at least one compound selected from salivary components involved in metabolism in the buccal cavity or in regulating the pH of the buccal cavity selected from the list consisting of Phosphate, Lactate and Formate;
(b) comparing a response variable with a corresponding reference response variable in a population of subjects without caries or comparing a response variable with a corresponding reference response variable in a population of subjects with caries, wherein a response variable is calculated from at least one of the concentrations determined in i) and at least one of the concentrations determined in ii) and at least one of the concentrations determined in iii) in step (a);
and
(c) determining the caries risk based on the degree of difference in response variable compared in step (b), wherein
a greater degree of difference between the response variable of the subject and the corresponding reference response variable in a population of subjects without caries, or
a smaller degree of difference between the response variable of the subject and the corresponding reference response variable in a population of subjects with caries,
is indicative of higher caries risk in said subject.

9 more elements

A kit for predicting caries risk in a subject characterized in that said kit comprises:

(a) means for determining the concentration in saliva from said subject of
i) at least one compound selected from salivary components of the immune system selected from the list consisting of Cathelicidin LL-37, IgA and Beta-Defensin-2;
and
ii) at least one compound selected from salivary components involved in microbial adhesion to a surface of the buccal cavity selected from the list consisting of Statherin, Fibronectin and Collagen I;
and
iii) at least one compound selected from salivary components involved in metabolism in the buccal cavity or in regulating the pH of the buccal cavity selected from the list consisting of Phosphate, Lactate and Formate,
wherein said means are selected from spectrophotometric, mass spectrometric, gravimetric, volumetric, calorimetric, electrochemical, microelectromechanical such as lab-on-a-chip and immunohistochemical techniques or instruments, or combinations thereof;
(b) instructions for comparing a response variable with a corresponding reference response variable in a population of subjects without caries or comparing a response variable with a corresponding reference response variable in a population of subjects with caries, wherein a response variable is calculated from at least one of the concentrations determined in i) and at least one of the concentrations determined in ii) and at least one of the concentrations determined in iii) in step (a);
and
(c) instructions for determining the caries risk based on the degree of difference in response variable compared in step (b), wherein
a greater degree of difference between the response variable of the subject and the corresponding reference response variable in a population of subjects without caries, or
a smaller degree of difference between the response variable of the subject and the corresponding reference response variable in a population of subjects with caries,
is indicative of higher caries risk in said subject.

10 more elements

Dependencies are inferred from the claim text.

In-vitro method and kit for diagnosing the risk of suffering from caries

Life of this patent

In-vitro method and kit for diagnosing the risk of suffering from caries

Life of this patent

Abstract

Claims11