Methods for analyzing nucleic acid sequences
AssigneeSynthego Corporation
InventorsTimothy HSIAU, Richard STONER, Travis MAURES, David CONANT
Life of this patent
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Abstract
The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.
Claims23
A method comprising determining, by a computer system, a plurality of predicted sequences of individual nucleic acid molecules in a sample contacted by at least two different nucleic acid editing tools based on a Sanger sequencing trace of a plurality of nucleic acid molecules from the sample contacted by the at least two different nucleic acid editing tools.
-60. (canceled)
A method comprising determining, by a computer system, a predicted knockout sequence of a single nucleic acid molecule based on a first Sanger sequencing trace and a second Sanger sequencing trace, wherein the predicted knockout sequence comprises an indel when compared to the second Sanger sequencing trace.
-101. (canceled)
-176. (canceled)
A method comprising determining, by a computer system, a first predicted sequence of a single nucleic acid molecule and a second predicted sequence of a single nucleic acid molecule, wherein the first and second predicted sequences are based on a first Sanger sequencing trace of a first plurality of nucleic acid molecules from a first sample, wherein the first and second predicted sequences each comprises an indel when compared to a second Sanger sequencing trace of a second plurality of nucleic acid molecules from a second sample, and wherein the indels of the first and second predicted sequences have a same size and a different nucleic acid sequence.
-215. (canceled)
Dependencies are inferred from the claim text.