Reagents and methods for detecting analytes
AssigneeAscensia Diabetes Care Holdings AG
InventorsAmy H. Chu, Karen L. Marfurt, Brenda L. Tudor, Mary Ellen Warchal-Windham, Boru Zhu
Life of this patent
Estimating the term…
Abstract
A reagent for detecting an analyte comprises a flavoprotein enzyme, a mediator such as a phenothiazine mediator, at least one surfactant, a polymer and a buffer. The reagent may be used with an electrochemical test sensor that includes a plurality of electrodes.
Claims27
A reagent for determining an analyte concentration, the reagent comprising:
- a flavoprotein enzyme;
- a 3-(2′,5′-disulfophenylimino)-3H-phenothiazine mediator;
- at least one surfactant;
- a hydroxyethyl cellulose polymer;
- and
- a buffer,
- wherein at least one of the mediator and the buffer includes an inorganic salt, and a ratio of the total inorganic salt to mediator is less than about 3:1.
(canceled)
(canceled)
A reagent for determining an analyte concentration in a fluid sample, the reagent comprising:
- FAD-glucose dehydrogenase having an activity of from about 0.1 Units/μL to about 10 Units/μL;
- a 3-(2′,5′-disulfophenylimino)-3H-phenothiazine mediator having a concentration of from about 5 mM to about 120 mM;
- a surfactant having a concentration of from about 0.05 wt. % to about 0.5 wt. % of the reagent;
- a hydroxyethyl cellulose polymer having a concentration of from about 0.1 wt. % to about 4 wt. % of the reagent;
- and
- a buffer,
- wherein at least one of the mediator and the buffer includes an inorganic salt, and a ratio of the total inorganic salt to mediator is less than about 2:1.
12-21. (canceled)
A method of determining an analyte concentration in a fluid sample, the method comprising the acts of:
- providing an electrode surface;
- providing a reagent including a flavoprotein enzyme, a 3-(2′,5′-disulfophenylimino)-3H-phenothiazine mediator,
- at least one surfactant, a buffer, and a hydroxyethyl cellulose polymer, the reagent being in contact with the electrode surface;
- contacting the fluid sample with the reagent;
- and
- determining the concentration of the analyte.
23-24. (canceled)
26-27. (canceled)
Dependencies are inferred from the claim text.