Artificial secretion peptides for heterologous protein production
AssigneePresident and Fellows of Harvard College
InventorsAnik Debnath, George Church
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Abstract
Provided herein, in some embodiments, are artificial secretion peptides capable of directing secretion from Lactobacillus for use, for example, in producing heterologous proteins, including therapeutic proteins.
Claims32
An artificial secretion signal peptide comprising an amino acid sequence that has at least 90% identity to an amino acid sequence of any one of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:
- 11, and SEQ ID NO:7.
The nucleic acid of claim 22, wherein the nucleic acid comprises an inducible promoter operably linked to a nucleotide sequence encoding the protein.
A method, comprising:
- (a) computing an amino acid residue position weight matrix (PWM) for a population of signal sequences within a library of bacterial strain-specific sequences;
- and
- (b) generating a consensus signal peptide sequence based on the PWM for the population of signal sequences.
A method comprising administering to a subject having an inflammatory bowel disease an engineered Lactobacillus rhamnosus cell comprising a nucleic acid encoding an anti-inflammatory cytokine fused to an artificial secretion signal peptide derived from Lactobacillus rhamnosus.
Dependencies are inferred from the claim text.