Stabilized reducing agents and methods using same
Assignee10x Genomics, Inc.
InventorsLawrence Greenfield, Christopher Hindson
Life of this patent
Estimating the term…
Abstract
The disclosure provides stabilized reducing agents and methods for using them in sample preparation. Stabilized reducing agents described herein provide easy-to-use replacement reducing agents for reducing agents that undergo side-reactions that can render them ineffective as reducing agents and/or decrease the concentration of available reducing agent. In some cases, a stabilized reducing agent is an activatable reducing agent that can be activated upon application of a stimulus to the reducing agent.
Claims31
1-51. (canceled)
A method for processing a nucleic acid molecule, comprising:
- (a) providing a reaction volume comprising said nucleic acid molecule, a reagent, and an agent that comprises a sterically hindered —SH group, wherein said reagent comprises a first component coupled to a second component via a linker, and wherein said linker comprises a cleavable moiety;
- and
- (b) subjecting said reaction volume to conditions sufficient for said agent to cleave said cleavable moiety, thereby separating said first component from said second component.
A reaction volume comprising:
- a nucleic acid molecule, a reagent, and an agent that comprises a sterically hindered —SH group, wherein said reagent comprises a first component coupled to a second component via a linker, and wherein said linker comprises a cleavable moiety that is configured such that when said reaction volume is subjected to conditions sufficient for said agent to cleave said cleavable moiety, said first component separates from said second component.
A method for processing a nucleic acid molecule, comprising:
- (a) providing a reaction volume comprising said nucleic acid molecule, a reagent, and an agent in an inactive state, wherein said reagent comprises a first component coupled to a second component via a linker, wherein said agent comprises one or more —SH groups, and wherein said agent comprises a first cleavable moiety and said linker comprises a second cleavable moiety;
- and
- (b) subjecting said reaction volume to conditions sufficient to cleave said first cleavable moiety of said agent to provide an activated agent that cleaves said second cleavable moiety of said linker, thereby separating said first component from said second component.
A reaction volume comprising:
- a nucleic acid molecule, a reagent, and an agent in an inactive state, wherein said reagent comprises a first component coupled to a second component via a linker, wherein said agent comprises one or more —SH groups, and wherein said agent comprises a first cleavable moiety and said linker comprises a second cleavable moiety that are configured such that when said reaction volume is subjected to conditions sufficient for cleavage of said first cleavable moiety of said agent, an activated agent is provided that is configured to cleave said second cleavable moiety of said linker such that said first component separates from said second component.
Dependencies are inferred from the claim text.