Highly efficient enzymatic process to produce (r)-3-quinuclidinol

A process to produce (R)-3-quinuclidinol comprising enzymatic reduction of 3-quinuclidinone or salt thereof using a variant of ketoreductase enzyme derived from Rhodotorula rubra, in the presence of a co-factor regenerating system variant of glucose dehydrogenase derived from Bacillus megaterium;

• wherein both the variant are in cell lysate;
• and wherein substrate loading capacity of the variant of ketoreductase enzyme is not less than about 100 g/L.

A process to enzymatically produce (R)-3-quinuclidinol wherein (R)-3-quinuclidinol produced is not less than 99% pure and has greater than 99.5% enantiomeric excess, wherein the enzyme is in cell lysate;

• and wherein substrate loading capacity of the enzyme is not less than about 100 g/L.

(R)-3-quinuclidinol, produced by the process comprising enzymatic reduction of 3-quinuclidinone or salt thereof using a variant of ketoreductase enzyme derived from Rhodotorula rubra, in the presence of a co-factor regenerating system variant of glucose dehydrogenase derived from Bacillus megaterium wherein both the variant are in cell lysate;

• wherein substrate loading capacity of the variant of ketoreductase enzyme is not less than about 100 g/L;
• and the (R)-3-quinuclidinol is not less than 99% pure and having greater than 99.5% enantiomeric excess.