Modified thiolases capable of producing branched compounds and uses thereof
AssigneeRepsol, S.A.
InventorsVicente BERNAL SÁNCHEZ, Pamela TORRES SALAS, Antonio MORREALE DE LEÓN, Víctor BARRERA BURGOS, María del Mar GONZÁLEZ BARROSO, Fernando LÓPEZ GALLEGO, Federico GAGO BADENAS, Pedro Alejandro SÁNCHEZ MURCIA
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Abstract
The invention relates to mutant thiolases capable of producing branched compounds which are considered of particular interest in industry. The invention also relates to method for obtaining said branched product.
Claims25
A mutant thiolase capable of catalyzing the formation of a compound of formula (I)
- by the condensation of a compound of formula (II)
- with a compound of formula (III)
- wherein R1 and R2 are independently selected from the group consisting of linear or branched C1-C6 alkyl, linear or branched C2-C6 alkenyl, linear or branched C2-C6 alkynyl, aryl, aryl-alkyl and benzyl, and S-CoA is coenzyme A,
- said mutant thiolase having at least a mutation at a position corresponding to position 293 in the polypeptide of SEQ ID NO:
- 1 wherein the thiolase shows at least a 75% sequence identity with a thiolase selected from the group consisting of SEQ ID NO:1, 2, 3 or 4, with the proviso that said thiolase is not a thiolase consisting of the sequence SEQ ID NO:
- 7 or SEQ ID NO:
- 8.
(canceled)
(canceled)
10-20. (canceled)
A process for the production of a compound of formula (I) comprising contacting a compound of formula (II) and a compound of formula (III) in the presence of a mutant thiolase capable of catalyzing the formation of a compound of formula (I)
- by the condensation of a compound of formula (II)
- with a compound of formula (III)
- wherein R1 and R2 are independently selected from the group consisting of linear or branched C1-C6 alkyl, linear or branched C2-C6 alkenyl, linear or branched C2-C6 alkynyl, aryl, aryl-alkyl and benzyl, and S-CoA is coenzyme A,
- said mutant thiolase having at least a mutation with respect to the naturally-occurring thiolase, wherein said mutation is located at a position corresponding to position 293 in the polypeptide of SEQ ID NO:
- 1;
- and wherein said contacting is carried out under conditions adequate for the condensation of compounds of formula (II) and (III) into a compound of formula (I).
32-42. (canceled)
45-46. (canceled)
Dependencies are inferred from the claim text.