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US 2020/0270601 A1Published application
Automated cell processing methods, modules, instruments, and systems
AssigneeInscripta, Inc.
InventorsPhillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill, Kevin Ness
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Abstract
In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
Claims20
An automated stand-alone multi-module cell editing instrument comprising:
- a housing configured to house all or some of the modules;
- a receptacle configured to receive cells;
- a receptacle configured to receive nucleic acids;
- a growth module for growing cells for transformation, wherein the growth module comprises a growth vial comprising one or more paddles;
- a transformation module configured to introduce the nucleic acids into the cells;
- a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells;
- a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script;
- and
- an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention.
6 more elements
An automated stand-alone multi-module cell editing instrument comprising:
- a housing configured to house some or all of the modules;
- a receptacle configured to receive cells;
- at least one receptacle configured to receive nucleic acids for desired genome editing events in the cells;
- a growth module configured to grow the cells;
- a cell wash and concentration module;
- a transformation module;
- a nuclease-directed editing module configured to allow the assembled nucleic acids to edit nucleic acids in the cells;
- a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a script;
- and
- an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the cell wash and concentration module, from the cell wash and concentration module to the transformation module, and from the transformation module to the nuclease-directed editing module, as well as from the nucleic acid receptacle to the transformation module, all without user intervention.
7 more elements
Dependencies are inferred from the claim text.