Immunogenic peptides and their use in transplantation
AssigneesLife Sciences Research Partners VZW, Katholieke Universiteit Leuven
InventorJean-Marie SAINT-REMY
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Abstract
The present invention relates to the use of immunogenic peptides comprising a T-cell epitope derived from an allograft antigen and a redox motif such as C-(X)2-[CST] (SEQ ID NO: 18) or [CST]-(X)2-C(SEQ ID NO: 19) in the prevention and/or treatment of allograft rejection and in the manufacture of medicaments therefore.
Claims21
1-28. (canceled)
A method of preventing or treating in a recipient the rejection of a mammalian allograft, said method comprising administering a peptide comprising (i) a T-cell epitope derived from an alloantigenic protein of said allograft and comprising (ii) a C-XX-[CST] (SEQ ID NO:
- 18) or [CST]-XX-C(SEQ ID NO:
- 19) motif.
A method for obtaining a population of allograft antigen-specific regulatory T cells with cytotoxic properties, the method comprising the steps of:
- providing peripheral blood cells;
- contacting said cells with an immunogenic peptide comprising (i) a T-cell epitope derived from an allograft antigenic protein and (ii) a C-(X)2-[CST] (SEQ ID NO:
- 18) or [CST]-(X)2-C(SEQ ID NO:
- 19) motif;
- and
- expanding said cells in the presence of IL-2.
An isolated immunogenic peptide with a length of between 12 and 75 amino acids comprising:
- (i) an MHC class II T-cell epitope of an alloantigenic protein of an allograft, wherein said alloantigenic protein does not comprise C-XX-[CST] (SEQ ID NO:
- 18) or [CST]-XX-C(SEQ ID NO:
- 19) within 11 amino acids N- or C-terminally of said epitope in said alloantigenic protein, and
- (ii) a redox motif, which is C-(X)2-[CST] (SEQ ID NO:
- 18) or [CST]-(X)2-C(SEQ ID NO:
- 19);
- wherein said epitope and said motif are immediately adjacent to each other or are separated by at most 7 amino acids.
The peptide according to claim 44, wherein said alloantigenic protein does not comprise said motif.
Dependencies are inferred from the claim text.